Composite construction’s performance depends greatly on shear effectively transfer between steel and concrete, we usually set shear connector between surface of the two different materials, the connector is a determining factor of combinable capability. Composite open-web plate frame is a new type of composite construction, has good performance in application of large span and large load floor or roof. This paper carries on research on two kind of new shear connectors of the composite open-web plate frame, the shell type shear connector and the block type shear connector, and carried on push-out test, also done vertical pseudo-static analysis on composite open-web plate frame which adapt new connector, study the hysteretic behavior ultimate bearing capacity and damage form. Study shows that, block type shear connector which set additional steel bars has good mechanical properties, it’s a shear  connector; Composite construction which adapt the connector has good capability of energy consumption and higher safety storage under vertical load.